Publications from UCLA

35. Zhou, H., Xie, X., Tang, Y.* “Engineering Natural Products Using Combinatorial Biosynthesis and Biocatalysis.” Curr. Opin. Biotechnol., 2008, submitted.

34. Pickens, L. B., Tang, Y.* “Decoding and Engineering Tetracycline Biosynthesis.” Metab. Eng. 2008, submitted.

33. Zhang, W., Li, Y., Tang, Y.*, 2008, submitted.

32. Xie, X., Pashkov, I., Xue, G., Guerrero, J., Yeates, T., Tang, Y.* “Rational Improvement of Simvastatin Synthase Solubility in Escherichia coli Leads to Higher Whole-cell Biocatalytic Activity.” Biotechnol. Bioeng. 2008, in press. Link

31. Zhan, J., Watanabe, K., Tang, Y.* “Synergistic Actions of Monooxygenase and Cyclases in Aromatic Polyketide Biosynthesis.” ChembioChem 2008, 9, 1710 – 1715. Link

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30. Zhang, W., Watanabe, K., Cai, X., Jung, M. E., Tang, Y.*, Zhan, J.* “Identifying the Minimal Enzymes Required for Anhydrotetracycline Biosynthesis.” J. Am. Chem. Soc.  2008, 130, 6068-6069. Link

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29. Zhou, H., Zhan, J., Watanabe, K., Xie, X., Tang, Y.* “A Polyketide Macrolactone Synthase from the Filamentous Fungus Gibberella zeae.” Proc. Natl. Acad. Sci. USA, 2008, 105, 6249-6254. Link

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28. Ames, B., Korman, T., Zhang, W., Smith, P., Vi, T., Tang, Y., Tsai, S-C. “Crystal Structure and Functional Analysis of Tetracenomycin ARO/CYC: Implications for Cyclization Specificity of Aromatic Polyketides.” Proc. Natl. Acad. Sci. USA, 2008, 105, 5349-5354. Link

27. Ma, S. M.*, Zhan, J., Xie, X., Watanabe, K., Tang, Y.*, Zhang, W.* “Redirecting the Cyclization Steps of Fungal Polyketide Synthase.” J. Am. Chem. Soc. 2008, 130, 38-39. Link

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26. Zhang, W., Tang, Y.* “Combinatorial Biosynthesis of Natural Products” J. Med Chem. 2008, 51, 2629-2633. Link

25. Ma, S. M., Zhan, J., Watanabe, K., Xie, X., Zhang, W., Wang, C. C., Tang, Y.* “Enzymatic Synthesis of Aromatic Polyketides using PKS4 from Gibberella fujikuroi.” J. Am. Chem. Soc. 2007, 129, 10642-10643. Link

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24. Zhang, W.; Wilke, B. I.; Zhan, J.; Watanabe, K.; Boddy, C. N.*; Tang, Y.*  “A New Mechanism for Benzopyrone Formation in Aromatic Polyketide Biosynthesis.” J. Am. Chem. Soc. 2007, 129, 9304-9305. Link.

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23. Zhang, W., Watanabe. K., Wang, C. C. C., Tang, Y.* “Investigation of Early Tailoring Reactions in the Oxytetracycline Biosynthetic Pathway.” J. Biol. Chem. 2007, 282, 25717-25725. Link.

22. Xie, X., Wong, W. W., Tang, Y.* “Improving Simvastatin Bioconversion in Escherichia coli by Deletion of bioH.” Metab. Eng.  2007, 9, 379-386. Link

21. Ma, S. M., Tang, Y.* “Biochemical Characterization of the Minimal Polyketide Synthase Domains in the Lovastatin Nonaketide Synthase LovB.” FEBS J. 2007, 274, 2854–2864. Link  

20. Xie, X., Tang, Y.* “Efficient Synthesis of Simvastatin Using Whole-Cell Biocatalysis.” Appl. Environ. Microb. 2007, 73, 2054-2060.  Link

19. Zhang, W., Watanabe, K., Wang, C. C. C., Tang, Y.* “Heterologous Biosynthesis of Amidated Polyketides with Novel Cyclization Regioselectivity from Oxytetracycline Polyketide Synthase” J. Nat. Prod. 2006, 69, 1633-1636. Link

18. Xie, X., Watanabe, K., Wojcicki, W. A., Wang, C. C. C., Tang, Y.* “Biosynthesis of lovastatin analogs with a broadly specific acyltransferase.” Chem. Biol. 2006, 13, 1161-1169. Link

17. Zhang, W., Ames, B., Tsai, S-C., Tang, Y.*, "Tetracycline Biosynthesis: Sequencing of the Oxytetracycline Gene Cluster and Reconstitution of the Malonamyl-Specific Initiation module." Appl. Environ. Microb. 2006, 72, 2573-2580.  link

 

Publication from Postdoctoral Work (*: equal contribution authors)

16. Tang, Y. Lee, H.Y., Tang, Y., Kim, C-Y., Mathews, I. and Khosla, C. “Structural and Functional Studies on SCO1815: A -Ketoacyl-Acyl Carrier Protein Reductase from Streptomyces coelicolor A3(2).” Biochemistry, 2006, 45, 14085-14093.

15. Lee. T. S.*, Khosla, C., Tang, Y.* "Orthogonal Protein Interactions in Spore Pigment Producing and Antibiotic Producing Type II Polyketide Synthases." J. Antibiotics, 2005, 58, 664-667.

14. Lee. T. S.*, Khosla, C., Tang, Y.* "Engineered Biosynthesis of Aklanonic Acid Analogs." J. Am. Chem. Soc., 2005, 127, 12254-12262.

13. Khosla, C., Tang, Y., "Chemistry. A new route to designer antibiotics." Science 2005, 308, 367-368.

12. Tang, Y., Koppisch, A., Khosla, C. "The Acyltransferase Homolog Found in Initiation Modules of Type II PKS is an Acetyl-ACP Thiolase that Suppresses Acetate-Priming of the KS-CLF." Biochemistry 2004, 43, 9546-9555.

11. Tang, Y., Lee, T. S., Lee, H. Y., Khosla, C. "Exploring the Biosynthetic Potential of Bimodular Aromatic Polyketide Synthases." Tetrahedron, 2004, 7659-7671.

10. Kumar, P.*, Khosla, C., Tang, Y.* "Manipulation and Analysis of Polyketide Synthases." Methods in Enzymology Volume 388: Protein Engineering 2004, 269-293.

9. Tang, Y., Lee, T. S., Khosla, C. "Engineered Biosynthesis of Regioselectively Modified Aromatic Polyketides Using Bimodular Polyketide Synthases." PLoS Biol. 2004, 2, 227-237.

8. Tang, Y., Tsai, S. C., Khosla, C. "Polyketide Chain Length Control by Chain Length Factor." J. Am. Chem. Soc. 2003, 125, 12708-12709.

7. Tang, Y., Lee, T. S., Kobayashi, S., Khosla, C. "Ketosynthases in the Initiation and Elongation Modules of Aromatic Polyketide Synthases Have Orthogonal Acyl-Carrier Protein Specificity." Biochemistry 2003, 42, 6588-6595.

 

Publication from Graduate Work

6. Cirino, P.C , Tang, Y., Takahashi, K., Tirrell, D. A., Arnold, F. H. "Global Incorporation of Norleucine in Place of Methionine in Cytochrome P450 BM-3 Heme Domain Increases Peroxygenase Activity." Biotechnol Bioeng. 2003, 20, 729-734.

5. Wang, P., Tang, Y., Tirrell, D. A., "Incorporation of Trifluoroisoleucine into Proteins In Vivo." J. Am. Chem. Soc. 2003, 125, 6900-6906.

4. Tang, Y., Tirrell, D. A. "Attenuation of Leucyl-tRNA Synthetase Proofreading Activity in Escherichia coli Allows Introduction of An Array of Unnatural Amino Acids in vivo." Biochemistry 2002, 41, 10635-10645.

3. Tang, Y., Tirrell, D. A. "Biosynthesis of Highly Stable Coiled-coils Containing Hexafluoroleucine Using an Engineered Bacterial Host." J. Am. Chem. Soc. 2001, 123, 11089-11090.

2. Tang, Y., Ghirlanda, G., Petka, W. A., Nakajima, T., W. A., DeGrado, W. F., Tirrell, D. A. "Fluorinated Coiled-coils Prepared in vivo Display Enhanced Thermal and Chemical Stability." Angew. Chem. Int. Ed. 2001, 40, 1494-1497.

1. Tang, Y., Ghirlanda, G., Vaidehi, N., Kua, J., Mainz, D. T., Goddard, W. A., DeGrado, W. F., Tirrell, D. A. "Stabilization of Leucine Zipper Coiled-coils using Trifluoroleucine." Biochemistry 2001, 40, 2790-2796.

 

Book Chapters

4. Zhang, W., Ferreira, J. P., Tang, Y. "Regulation of Secondary Metabolism Biosynthesis." In: Smolke, C.D., ed. Handbook for Metabolic Pathway Engineering. CRC Press. 2006 Submitted.

3. Tang, Y.; Ma, S.M; Wojcicki, W. A. "Applications of Metabolic Engineering for Natural Drug Discovery." In: Smolke, C.D., ed. Handbook for Metabolic Pathway Engineering. CRC Press. 2006 Submitted.

2. Zhang, W., Tang, Y., "Engineering Starter Units in Aromatic Polyketides." In ACS Volume Based on Polyketides: Biosynthesis, Biological Activity and Genetic Engineering. eds S. R. Baerson; American Chemical Society, 2006.

1. Tang, Y., Khosla, C., "Diversity from Mother Nature: natural and unnatural products." In Exploiting Chemical Diversity for Drug Discovery, eds P. Bartlett and M. Entzeroth; Royal Society of Chemistry, 2006. Link

 

 

 

 

 

Copyright 2007