A water droplet coalescing into a pool of methanol. Because of the large difference in surface tension, the water droplet pulls the interface up its sides and causes a pinch off of a secondary drop to happen far above the methanol/air interface.
Nikon TIRF System
This imaging system utilizes an evanescent wave to excite single molecules in the thin section in contact with the coverglass.
Image under TIRF observation
Image under epi-florescence observation
UCLA, Mechanical and Aerospace Engineering Department