Publications from UCLA

45. Gu, Z., Yan, M., Hu, B., Joo, K., Biswas, A., Lu, Y.*, Wang, P.*, Tang, Y.* “Protein Nanocapsules Weaved by an Enzymatically Degradable Polymer Network.” Nano. Lett. 2009, in press. Link

44. Gao, X., Xie, X., Pashkov, I., Sawaya, R. M., Laidmen, J., Zhang, W., Cacho, R., Yeates, T. O.*, Tang, Y.* “Directed Evolution and Structural Characterization of a Simvastatin Synthase.” Chem. Biol. 2009, 16, 1064-1074. Link

43. Pickens, L. B., Kim, W., Wang, P., Zhou, H., Watanabe, K., Gomi, S., Tang, Y.* “Biochemical Analysis of the Biosynthetic Pathway of an Anticancer Tetracycline SF2575.” J. Am. Chem. Soc. 2009, in press. Link

42. Ma, S. A., Li, J., Choi, J. W., Zhou, H., Lee, M., Moorthie, V., Xie, X., Kealey, J. T., Da Silva, N. A., Vederas, J. C.*, Tang, Y.* “Complete Reconstitution of a Highly-Reducing Iterative Polyketide Synthase.” Science 2009, 326, 589-592. Link

41. Wang, M., Zhou, H., Wirz, M., Tang, Y., Boddy, C. N.* “A Versatile Thioesterase from an Iterative Fungal Polyketide Synthase.” Biochemistry 2009, 48, 6288-6290. Link

40. Xie, X., Meehan, M. J. Xu, W., Dorrestein, P. C., Tang, Y.* “Acyltransferase Mediated Polyketide Release From Fungal Megasynthase.” J. Am. Chem. Soc. 2009, 131, 8388-8389. Link

39. Wang, P., Zhang, W., Zhan, J., Tang, Y.* “Identification of OxyE as an Ancillary Oxygenase During Tetracycline Biosynthesis.” ChemBioChem, 2009, 10, 1554-1550. Link

38. Zhan, J., Qiao, K., Tang, Y.* “Investigation of Post-PKS Tailoring Modifications in Pradimicin Biosynthesis.” ChemBioChem, 2009, 10, 1447-1452. Link

37. Ma, S. M., Pickens, L. B., Tang, Y.* “Antibiotics, Polyketides.” Encyclopedia of Industrial Biotechnology, 2009, in press.

36. Zhang, W., Tang, Y.^* “In vitro Analysis of Type II Polyketides.” Methods in Enzymology Volume: Complex Enzymes in Microbial Natural Product Biosynthesis. 2009, 459, 367-393. Link

35. Pickens, L. B., Tang, Y. ^* “Decoding and Engineering Tetracycline Biosynthesis.” Metab. Eng. 2009, 11, 69-75. (Cover article). Link

34. Xie, X., Pashkov, I., Xue, G., Guerrero, J., Yeates, T., Tang, Y.* “Rational Improvement of Simvastatin Synthase Solubility in Escherichia coli Leads to Higher Whole-cell Biocatalytic Activity.” Biotechnol. Bioeng. 2009, 102, 20-28. Link

33. Zhang, W., Li, Y., Tang, Y. “Engineered Biosynthesis of Bacterial Aromatic Polyketides in Escherichia coli.” Proc. Natl. Acad. Sci. USA., 2008, 105, 20683-20685. Link

32. Zhou, H., Xie, X., Tang, Y.* “Engineering Natural Products Using Combinatorial Biosynthesis and Biocatalysis.” Curr. Opin. Biotechnol., 2008, 19, 590-596. Link

31. Zhan, J., Watanabe, K., Tang, Y.* “Synergistic Actions of Monooxygenase and Cyclases in Aromatic Polyketide Biosynthesis.” ChembioChem 2008, 9, 1710 – 1715. Link

30. Zhang, W., Watanabe, K., Cai, X., Jung, M. E., Tang, Y.*, Zhan, J.* “Identifying the Minimal Enzymes Required for Anhydrotetracycline Biosynthesis.” J. Am. Chem. Soc. 2008, 130, 6068-6069. Link

29. Zhou, H., Zhan, J., Watanabe, K., Xie, X., Tang, Y.* “A Polyketide Macrolactone Synthase from the Filamentous Fungus Gibberella zeae.” Proc. Natl. Acad. Sci. USA, 2008, 105, 6249-6254. Link

28. Ames, B., Korman, T., Zhang, W., Smith, P., Vi, T., Tang, Y., Tsai, S-C. “Crystal Structure and Functional Analysis of Tetracenomycin ARO/CYC: Implications for Cyclization Specificity of Aromatic Polyketides.” Proc. Natl. Acad. Sci. USA, 2008, 105, 5349-5354. Link

27. Ma, S. M.*, Zhan, J., Xie, X., Watanabe, K., Tang, Y.*, Zhang, W.* “Redirecting the Cyclization Steps of Fungal Polyketide Synthase.” J. Am. Chem. Soc. 2008, 130, 38-39. Link

26. Zhang, W., Tang, Y.* “Combinatorial Biosynthesis of Natural Products” J. Med Chem. 2008, 51, 2629-2633. Link

25. Ma, S. M., Zhan, J., Watanabe, K., Xie, X., Zhang, W., Wang, C. C., Tang, Y.* “Enzymatic Synthesis of Aromatic Polyketides using PKS4 from Gibberella fujikuroi.” J. Am. Chem. Soc. 2007, 129, 10642-10643. Link

24. Zhang, W.; Wilke, B. I.; Zhan, J.; Watanabe, K.; Boddy, C. N.*; Tang, Y.* “A New Mechanism for Benzopyrone Formation in Aromatic Polyketide Biosynthesis.” J. Am. Chem. Soc. 2007, 129, 9304-9305. Link.

23. Zhang, W., Watanabe. K., Wang, C. C. C., Tang, Y.* “Investigation of Early Tailoring Reactions in the Oxytetracycline Biosynthetic Pathway.” J. Biol. Chem. 2007, 282, 25717-25725. Link.

22. Xie, X., Wong, W. W., Tang, Y.* “Improving Simvastatin Bioconversion in Escherichia coli by Deletion of bioH.” Metab. Eng. 2007, 9, 379-386. Link

21. Ma, S. M., Tang, Y.* “Biochemical Characterization of the Minimal Polyketide Synthase Domains in the Lovastatin Nonaketide Synthase LovB.” FEBS J. 2007, 274, 2854–2864. Link

20. Xie, X., Tang, Y.* “Efficient Synthesis of Simvastatin Using Whole-Cell Biocatalysis.” Appl. Environ. Microb. 2007, 73, 2054-2060. Link

19. Zhang, W., Watanabe, K., Wang, C. C. C., Tang, Y.* “Heterologous Biosynthesis of Amidated Polyketides with Novel Cyclization Regioselectivity from Oxytetracycline Polyketide Synthase” J. Nat. Prod. 2006, 69, 1633-1636. Link

18. Xie, X., Watanabe, K., Wojcicki, W. A., Wang, C. C. C., Tang, Y.* “Biosynthesis of lovastatin analogs with a broadly specific acyltransferase.” Chem. Biol. 2006, 13, 1161-1169. Link

17. Zhang, W., Ames, B., Tsai, S-C., Tang, Y.*, "Tetracycline Biosynthesis: Sequencing of the Oxytetracycline Gene Cluster and Reconstitution of the Malonamyl-Specific Initiation module." Appl. Environ. Microb. 2006, 72, 2573-2580. link

Publication from Postdoctoral Work

16. Tang, Y. Lee, H.Y., Tang, Y., Kim, C-Y., Mathews, I. and Khosla, C. “Structural and Functional Studies on SCO1815: A -Ketoacyl-Acyl Carrier Protein Reductase from Streptomyces coelicolor A3(2).” Biochemistry, 2006, 45, 14085-14093.

15. Lee. T. S.*, Khosla, C., Tang, Y.* "Orthogonal Protein Interactions in Spore Pigment Producing and Antibiotic Producing Type II Polyketide Synthases." J. Antibiotics, 2005, 58, 664-667.

14. Lee. T. S.*, Khosla, C., Tang, Y.* "Engineered Biosynthesis of Aklanonic Acid Analogs." J. Am. Chem. Soc., 2005, 127, 12254-12262.

13. Khosla, C., Tang, Y., "Chemistry. A new route to designer antibiotics." Science 2005, 308, 367-368.

12. Tang, Y., Koppisch, A., Khosla, C. "The Acyltransferase Homolog Found in Initiation Modules of Type II PKS is an Acetyl-ACP Thiolase that Suppresses Acetate-Priming of the KS-CLF." Biochemistry 2004, 43, 9546-9555.

11. Tang, Y., Lee, T. S., Lee, H. Y., Khosla, C. "Exploring the Biosynthetic Potential of Bimodular Aromatic Polyketide Synthases." Tetrahedron, 2004, 7659-7671.

10. Kumar, P.*, Khosla, C., Tang, Y.* "Manipulation and Analysis of Polyketide Synthases." Methods in Enzymology Volume 388: Protein Engineering 2004, 269-293.

9. Tang, Y., Lee, T. S., Khosla, C. "Engineered Biosynthesis of Regioselectively Modified Aromatic Polyketides Using Bimodular Polyketide Synthases." PLoS Biol. 2004, 2, 227-237.

8. Tang, Y., Tsai, S. C., Khosla, C. "Polyketide Chain Length Control by Chain Length Factor." J. Am. Chem. Soc. 2003, 125, 12708-12709.

7. Tang, Y., Lee, T. S., Kobayashi, S., Khosla, C. "Ketosynthases in the Initiation and Elongation Modules of Aromatic Polyketide Synthases Have Orthogonal Acyl-Carrier Protein Specificity." Biochemistry 2003, 42, 6588-6595.

Publication from Graduate Work

6. Cirino, P.C , Tang, Y., Takahashi, K., Tirrell, D. A., Arnold, F. H. "Global Incorporation of Norleucine in Place of Methionine in Cytochrome P450 BM-3 Heme Domain Increases Peroxygenase Activity." Biotechnol Bioeng. 2003, 20, 729-734.

5. Wang, P., Tang, Y., Tirrell, D. A., "Incorporation of Trifluoroisoleucine into Proteins In Vivo." J. Am. Chem. Soc. 2003, 125, 6900-6906.

4. Tang, Y., Tirrell, D. A. "Attenuation of Leucyl-tRNA Synthetase Proofreading Activity in Escherichia coli Allows Introduction of An Array of Unnatural Amino Acids in vivo." Biochemistry 2002, 41, 10635-10645.

3. Tang, Y., Tirrell, D. A. "Biosynthesis of Highly Stable Coiled-coils Containing Hexafluoroleucine Using an Engineered Bacterial Host." J. Am. Chem. Soc. 2001, 123, 11089-11090.

2. Tang, Y., Ghirlanda, G., Petka, W. A., Nakajima, T., W. A., DeGrado, W. F., Tirrell, D. A. "Fluorinated Coiled-coils Prepared in vivo Display Enhanced Thermal and Chemical Stability." Angew. Chem. Int. Ed. 2001, 40, 1494-1497.

1. Tang, Y., Ghirlanda, G., Vaidehi, N., Kua, J., Mainz, D. T., Goddard, W. A., DeGrado, W. F., Tirrell, D. A. "Stabilization of Leucine Zipper Coiled-coils using Trifluoroleucine." Biochemistry 2001, 40, 2790-2796.

Book Chapters

4. Zhang, W., Ferreira, J. P., Tang, Y. "Regulation of Secondary Metabolism Biosynthesis." In: Smolke, C.D., ed. Handbook for Metabolic Pathway Engineering. CRC Press. 2009 in press.

3. Tang, Y.; Ma, S.M; Wojcicki, W. A. "Applications of Metabolic Engineering for Natural Drug Discovery." In: Smolke, C.D., ed. Handbook for Metabolic Pathway Engineering. CRC Press. 2009 in press.

2. Zhang, W., Tang, Y., "Engineering Starter Units in Aromatic Polyketides." In ACS Volume Based on Polyketides: Biosynthesis, Biological Activity and Genetic Engineering. eds S. R. Baerson; American Chemical Society, 2006.

1. Tang, Y., Khosla, C., "Diversity from Mother Nature: natural and unnatural products." In Exploiting Chemical Diversity for Drug Discovery, eds P. Bartlett and M. Entzeroth; Royal Society of Chemistry, 2006. Link

Copyright 2007